Simultaneous Transmit and Receive operation requires a high amount of transmit-to-receive isolation in order to avoid self-interference. This isolation is best achieved by utilizing multiple cancellation techniques. The combination of adaptive multiple-input multiple-output spatial cancellation with a high-isolation antenna and RF canceller produces a novel system architecture that focuses on cancellation in the analog domain before the receiver's low-noise amplifier. A prototype of this system has been implemented on a moving vehicle, and measurements have proven that this design is capable of providing more than 90 dB of total isolation in realistic multi path environments over a 30 MHz bandwidth centered at 2.45 GHz. Index Terms-Adaptive systems, full-duplex wireless communication, interference cancellation, multiaccess communication, simultaneous transmit and receive, STAR.